A Set of Aspartyl Protease-Deficient Strains for Improved Expression of Heterologous Proteins in Kluyveromyces Lactis

FEMS Yeast Res. 2011 Mar;11(2):168-78. doi: 10.1111/j.1567-1364.2010.00703.x. Epub 2010 Dec 17.

Abstract

Secretion of recombinant proteins is a common strategy for heterologous protein expression using the yeast Kluyveromyces lactis. However, a common problem is degradation of a target recombinant protein by secretory pathway aspartyl proteases. In this study, we identified five putative pfam00026 aspartyl proteases encoded by the K. lactis genome. A set of selectable marker-free protease deletion mutants was constructed in the prototrophic K. lactis GG799 industrial expression strain background using a PCR-based dominant marker recycling method based on the Aspergillus nidulans acetamidase gene (amdS). Each mutant was assessed for its secretion of protease activity, its health and growth characteristics, and its ability to efficiently produce heterologous proteins. In particular, despite having a longer lag phase and slower growth compared with the other mutants, a Δyps1 mutant demonstrated marked improvement in both the yield and the quality of Gaussia princeps luciferase and the human chimeric interferon Hy3, two proteins that experienced significant proteolysis when secreted from the wild-type parent strain.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Arecaceae / enzymology
  • Aspartic Acid Proteases / deficiency*
  • DNA, Fungal / chemistry
  • DNA, Fungal / genetics
  • Fungal Proteins / genetics
  • Gene Deletion
  • Gene Expression*
  • Kluyveromyces / enzymology*
  • Kluyveromyces / genetics
  • Kluyveromyces / metabolism*
  • Luciferases / metabolism
  • Molecular Sequence Data
  • Mutagenesis
  • Recombinant Proteins / metabolism*
  • Sequence Analysis, DNA

Substances

  • DNA, Fungal
  • Fungal Proteins
  • Recombinant Proteins
  • Luciferases
  • Aspartic Acid Proteases

Associated data

  • GENBANK/HM015509