Early changes in hepatic redox homeostasis following treatment with a single dose of valproic acid

Biochem Pharmacol. 1990 Aug 1;40(3):589-93. doi: 10.1016/0006-2952(90)90560-8.


Changes in reduced glutathione (GSH) and pyridine nucleotide phosphate levels as well as in the activities of the glutathione peroxidase-reductase system and glucose-6-phosphate dehydrogenase have been studied in rats after a single i.p. administration of various doses of valproic acid (VPA). GSH level decreased in a dose-dependent relation. At the end of 180 min GSH levels either returned to control limits (lower doses) or showed a tendency to normalize (higher doses). GSH loss was paralleled by the reduction in glutathione reductase activity. A significant NADPH reduction was also seen after animal exposure to high VPA doses. At the end of 180 min a maximal NADPH decrease was reached. The activities of both glutathione peroxidase and glucose-6-phosphate dehydrogenase were suppressed irrespective of whether animals were given low or high VPA doses.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Female
  • Glucosephosphate Dehydrogenase / metabolism
  • Glutathione / metabolism*
  • Glutathione Peroxidase / metabolism
  • Glutathione Reductase / metabolism
  • Homeostasis / drug effects
  • Kinetics
  • Liver / enzymology*
  • NADP / metabolism*
  • Oxidation-Reduction
  • Rats
  • Valproic Acid / pharmacology*


  • NADP
  • Valproic Acid
  • Glucosephosphate Dehydrogenase
  • Glutathione Peroxidase
  • Glutathione Reductase
  • Glutathione