Contamination of clinical specimens with MLV-encoding nucleic acids: implications for XMRV and other candidate human retroviruses

Retrovirology. 2010 Dec 20;7:112. doi: 10.1186/1742-4690-7-112.

Abstract

Efforts to assess the prevalence of xenotropic murine leukemia virus-related virus (XMRV) in patients with prostate cancer and chronic fatigue syndrome have relied heavily on PCR-based testing of clinical samples and have yielded widely divergent findings. This week in Retrovirology, reports from four independent research groups illustrate the extreme care needed to exclude DNA or RNA contamination in PCR analyses of XMRV. In addition, phylogenetic evidence suggesting that previously-published XMRV sequences originated from a commonly-used prostate carcinoma cell line (22Rv1) is presented. These findings raise important questions regarding the provenance of XMRV and its potential connection to human disease.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA Contamination*
  • Fatigue Syndrome, Chronic / genetics
  • Fatigue Syndrome, Chronic / virology
  • Humans
  • Leukemia Virus, Murine / genetics
  • Leukemia Virus, Murine / isolation & purification*
  • Male
  • Nucleic Acids / analysis
  • Nucleic Acids / genetics
  • Prostatic Neoplasms / genetics
  • Prostatic Neoplasms / virology
  • Reagent Kits, Diagnostic / virology
  • Retroviridae / genetics
  • Xenotropic murine leukemia virus-related virus / genetics
  • Xenotropic murine leukemia virus-related virus / isolation & purification*

Substances

  • Nucleic Acids
  • Reagent Kits, Diagnostic