Fast tomographic reconstruction on multicore computers
- PMID: 21172911
- DOI: 10.1093/bioinformatics/btq692
Fast tomographic reconstruction on multicore computers
Abstract
Tomo3D implements a multithreaded vectorized approach to tomographic reconstruction that takes full advantage of the computer power in modern multicore computers. Full resolution tomograms are generated at high speed on standard computers with no special system requirements. Tomo3D has the most common reconstruction methods implemented, namely weighted Back-projection (WBP) and simultaneous iterative reconstruction technique (SIRT). It proves to be competitive with current graphic processor unit solutions in terms of processing time, in the order of a few seconds with WBP or minutes with SIRT. The program is compatible with standard packages, which easily allows integration in the electron tomography workflow.
Similar articles
-
Tomo3D 2.0--exploitation of advanced vector extensions (AVX) for 3D reconstruction.J Struct Biol. 2015 Feb;189(2):147-52. doi: 10.1016/j.jsb.2014.11.009. Epub 2014 Dec 17. J Struct Biol. 2015. PMID: 25528570
-
Vectorization with SIMD extensions speeds up reconstruction in electron tomography.J Struct Biol. 2010 Jun;170(3):570-5. doi: 10.1016/j.jsb.2010.01.008. Epub 2010 Jan 18. J Struct Biol. 2010. PMID: 20085820
-
Evaluation of a multicore-optimized implementation for tomographic reconstruction.PLoS One. 2012;7(11):e48261. doi: 10.1371/journal.pone.0048261. Epub 2012 Nov 6. PLoS One. 2012. PMID: 23139768 Free PMC article.
-
Cryo-Electron Tomography and Subtomogram Averaging.Methods Enzymol. 2016;579:329-67. doi: 10.1016/bs.mie.2016.04.014. Epub 2016 Jun 22. Methods Enzymol. 2016. PMID: 27572733 Review.
-
Subtomogram averaging from cryo-electron tomograms.Methods Cell Biol. 2019;152:217-259. doi: 10.1016/bs.mcb.2019.04.003. Epub 2019 May 15. Methods Cell Biol. 2019. PMID: 31326022 Review.
Cited by
-
Automatic alignment and reconstruction of images for soft X-ray tomography.J Struct Biol. 2012 Feb;177(2):259-66. doi: 10.1016/j.jsb.2011.11.027. Epub 2011 Dec 2. J Struct Biol. 2012. PMID: 22155289 Free PMC article.
-
Resolving macromolecular structures from electron cryo-tomography data using subtomogram averaging in RELION.Nat Protoc. 2016 Nov;11(11):2054-65. doi: 10.1038/nprot.2016.124. Epub 2016 Sep 29. Nat Protoc. 2016. PMID: 27685097 Free PMC article.
-
Development of Correlative Cryo-soft X-ray Tomography and Stochastic Reconstruction Microscopy. A Study of Cholesterol Crystal Early Formation in Cells.J Am Chem Soc. 2016 Nov 16;138(45):14931-14940. doi: 10.1021/jacs.6b07584. Epub 2016 Nov 8. J Am Chem Soc. 2016. PMID: 27934213 Free PMC article.
-
XTEND: Extending the depth of field in cryo soft X-ray tomography.Sci Rep. 2017 Apr 4;7:45808. doi: 10.1038/srep45808. Sci Rep. 2017. PMID: 28374769 Free PMC article.
-
In vivo structures of an intact type VI secretion system revealed by electron cryotomography.EMBO Rep. 2017 Jul;18(7):1090-1099. doi: 10.15252/embr.201744072. Epub 2017 May 9. EMBO Rep. 2017. PMID: 28487352 Free PMC article.
Publication types
MeSH terms
LinkOut - more resources
Full Text Sources
