Three-gene predictor of clinical outcome for gastric cancer patients treated with chemotherapy

Pharmacogenomics J. 2012 Apr;12(2):119-27. doi: 10.1038/tpj.2010.87. Epub 2010 Dec 21.


To identify transcriptional profiles predictive of the clinical benefit of cisplatin and fluorouracil (CF) chemotherapy to gastric cancer patients, endoscopic biopsy samples from 96 CF-treated metastatic gastric cancer patients were prospectively collected before therapy and analyzed using high-throughput transcriptional profiling and array comparative genomic hybridization. Transcriptional profiling identified 917 genes that are correlated with poor patient survival after CF at P<0.05 (poor prognosis signature), in which protein synthesis and DNA replication/recombination/repair functional categories are enriched. A survival risk predictor was then constructed using genes, which are included in the poor prognosis signature and are contained within identified genomic amplicons. The combined expression of three genes-MYC, EGFR and FGFR2-was an independent predictor for overall survival of 27 CF-treated patients in the validation set (adjusted P=0.017), and also for survival of 40 chemotherapy-treated gastric cancer patients in a published data set (adjusted P=0.026). Thus, combined expression of MYC, EGFR and FGFR2 is predictive of poor survival in CF-treated metastatic gastric cancer patients.

Publication types

  • Research Support, N.I.H., Intramural
  • Research Support, Non-U.S. Gov't
  • Validation Study

MeSH terms

  • Aged
  • Antineoplastic Agents / therapeutic use*
  • ErbB Receptors / genetics*
  • Female
  • Genes, myc*
  • Humans
  • Male
  • Neoplasm Metastasis
  • Prognosis
  • Prospective Studies
  • Receptor, Fibroblast Growth Factor, Type 2 / genetics*
  • Stomach Neoplasms / drug therapy*
  • Stomach Neoplasms / genetics
  • Stomach Neoplasms / pathology
  • Survival Analysis
  • Treatment Outcome


  • Antineoplastic Agents
  • ErbB Receptors
  • FGFR2 protein, human
  • Receptor, Fibroblast Growth Factor, Type 2

Associated data

  • GEO/GSE14210