Up-regulation of the vitamin C transporter SVCT2 upon differentiation and depolarization of myotubes

FEBS Lett. 2011 Jan 21;585(2):390-6. doi: 10.1016/j.febslet.2010.12.020. Epub 2010 Dec 19.


In addition to its role as a strong antioxidant, vitamin C regulates the differentiation of several cell lineages. In vertebrate skeletal muscle, the vitamin C transporter SVCT2 is preferentially expressed in slow muscle fibers. To gain insights into the possible involvement of intracellular vitamin C on early myogenesis, we investigated the regulation of SVCT2 expression in cultures of chick fetal myoblasts. SVCT2 expression increases in cultures of both, slow and fast muscle-derived myoblasts, as they fuse to form mainly fast myotubes. Interestingly, we found that SVCT2 could be positively modulated by potassium-induced depolarization of myotubes. These findings suggest that SVCT2-mediated uptake of vitamin C could play diverse roles on skeletal muscle development and physiology.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Ascorbic Acid
  • Cell Differentiation*
  • Cell Polarity
  • Cells, Cultured
  • Chick Embryo
  • Chickens
  • Muscle Development
  • Muscle Fibers, Skeletal / chemistry
  • Muscle Fibers, Skeletal / cytology*
  • Myoblasts
  • Organic Anion Transporters, Sodium-Dependent / biosynthesis*
  • Sodium-Coupled Vitamin C Transporters
  • Symporters / biosynthesis*
  • Up-Regulation*


  • Organic Anion Transporters, Sodium-Dependent
  • Sodium-Coupled Vitamin C Transporters
  • Symporters
  • Ascorbic Acid