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, 6 (7), 834-44

Microbial and Bioconversion Production of D-xylitol and Its Detection and Application


Microbial and Bioconversion Production of D-xylitol and Its Detection and Application

Xi Chen et al. Int J Biol Sci.


D-Xylitol is found in low content as a natural constituent of many fruits and vegetables. It is a five-carbon sugar polyol and has been used as a food additive and sweetening agent to replace sucrose, especially for non-insulin dependent diabetics. It has multiple beneficial health effects, such as the prevention of dental caries, and acute otitis media. In industry, it has been produced by chemical reduction of D-xylose mainly from photosynthetic biomass hydrolysates. As an alternative method of chemical reduction, biosynthesis of D-xylitol has been focused on the metabolically engineered Saccharomyces cerevisiae and Candida strains. In order to detect D-xylitol in the production processes, several detection methods have been established, such as gas chromatography (GC)-based methods, high performance liquid chromatography (HPLC)-based methods, LC-MS methods, and capillary electrophoresis methods (CE). The advantages and disadvantages of these methods are compared in this review.

Keywords: Bioconversion production; Candida.; D-xylitol; Detection methods; Saccharomyces cerevisiae.

Conflict of interest statement

Conflict of Interests: The authors have declared that they have no conflict of interests.


Figure 1
Figure 1
Production of D-xylitol from photosynthetic biomass.
Figure 2
Figure 2
Metabolic pathway of D-xylitol in yeasts.

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