[Effect of astragalus membranaceus on the proliferation, osteogenic capacity and structure of periodontal ligament cells in vitro]

Hua Xi Kou Qiang Yi Xue Za Zhi. 2010 Oct;28(5):556-9.
[Article in Chinese]

Abstract

Objective: To investigate the effect of Astragalus membranaceus (APS) on the proliferation, osteogenic capacity and structure of periodontal ligament cells (PDLCs) in vitro.

Methods: PDLCs were cultured in vitro with APS of 0.08, 0.1, 0.2, 0.4 mg x mL(-1). Methyl thiazolyl tetrazolium (MTr), alkaline phosphatase (ALP) and cell structure were detected to determine the proliferation and differentiation of PDLCs proliferation and differentiation.

Results: When the APS was 0.2 mg x mL(-1), the absorbance of MTT and ALP exhibit significantly increased as compared to the control (P < 0.05). The cells cultured in vitro with APS of 0.2 mg x mL(-1) had the normal structure.

Conclusion: APS with proper concentration in short-term culture may promote the proliferation and differentiation of PDLCs.

MeSH terms

  • Alkaline Phosphatase
  • Astragalus propinquus*
  • Cell Differentiation
  • Cell Proliferation
  • In Vitro Techniques
  • Periodontal Ligament*

Substances

  • Alkaline Phosphatase