Aim and methods: To observe the effects of Puerarin on K+ channel of isolated ventricular myocyte in guinea pig.
Methods: Using inside-out configuration of patch-clamp single channel recording technique.
Results: Puerarin 20 micromol/L, 40 micromol/L, 80 microml/L could inhibit the open-close rate of K+ channel of isolated ventricular myocyte in guinea pig. At 80 micromol/L, Po was decreased from 0.867 +/- 0.13 to 0.019 +/- 0.01 (n = 5, P < 0.05).
Conclusion: Puerarin can inhibit K+ channel of isolated ventricular myocyte in guinea pig. It may be the mechanism of Puerarin against arrhythmias in molecular level.