The Blasticidin S Resistance Gene (Bsr) Selectable in a Single Copy State in the Bacillus Subtilis Chromosome

J Biochem. 1990 Jun;107(6):799-801. doi: 10.1093/oxfordjournals.jbchem.a123128.

Abstract

The blasticidin S resistance gene (bsr), originally isolated from Bacillus cereus, was studied in Bacillus subtilis. It was found that a 617 bp fragment including the intact bsr gene and its 5' flanking region could confer BS resistance on B. subtilis when integrated in its chromosome, even in a single copy state. The construction of a bsr gene cassette and its practical application as a novel selection marker for B. subtilis are reported.

MeSH terms

  • Antifungal Agents / pharmacology*
  • Bacillus subtilis / drug effects
  • Bacillus subtilis / genetics*
  • Bacillus subtilis / isolation & purification
  • Cloning, Molecular
  • Drug Resistance, Microbial / genetics
  • Genetic Markers
  • Nucleosides / pharmacology
  • Transformation, Bacterial*

Substances

  • Antifungal Agents
  • Genetic Markers
  • Nucleosides
  • blasticidin S