Measurement of 5-methyltetrahydrofolic acid in man by high-performance liquid chromatography

Metabolism. 1990 Sep;39(9):902-4. doi: 10.1016/0026-0495(90)90298-q.

Abstract

5-Methyl-5,6,7,8-tetrahydrofolic (5-methylTHF) acid can be measured in human serum and whole blood by fluorescence after deproteinization with perchloric acid and separation by high-performance liquid chromatography (HPLC). The technique is sensitive, rapid, and correlates with existing methods for total folates.

Publication types

  • Comparative Study

MeSH terms

  • Chromatography, High Pressure Liquid / methods
  • Humans
  • Indicators and Reagents
  • Lactobacillus casei / drug effects
  • Lactobacillus casei / growth & development
  • Spectrometry, Fluorescence
  • Tetrahydrofolates / blood*
  • Tetrahydrofolates / pharmacology

Substances

  • Indicators and Reagents
  • Tetrahydrofolates
  • 5-methyltetrahydrofolate