Coordinated induction of two unrelated glucose-regulated protein genes by a calcium ionophore: human BiP/GRP78 and GAPDH

Biochem Biophys Res Commun. 1990 Aug 31;171(1):431-8. doi: 10.1016/0006-291x(90)91411-k.

Abstract

The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Base Sequence
  • Blotting, Northern
  • Calcimycin / pharmacology*
  • Carrier Proteins / genetics*
  • Cell Line
  • Endoplasmic Reticulum Chaperone BiP
  • Gene Expression Regulation / drug effects*
  • Glyceraldehyde-3-Phosphate Dehydrogenases / genetics*
  • Heat-Shock Proteins*
  • Humans
  • In Vitro Techniques
  • Molecular Chaperones*
  • Molecular Sequence Data
  • Promoter Regions, Genetic
  • RNA, Messenger / genetics
  • Regulatory Sequences, Nucleic Acid

Substances

  • Carrier Proteins
  • Endoplasmic Reticulum Chaperone BiP
  • HSPA5 protein, human
  • Heat-Shock Proteins
  • Molecular Chaperones
  • RNA, Messenger
  • Calcimycin
  • Glyceraldehyde-3-Phosphate Dehydrogenases