Abstract
The induction of human BiP/GRP78 and GAPDH protein genes by the calcium ionophore A23187 was determined. Steady-state levels of mRNA for both the glucose starvation-responsive BiP/GRP78 gene and the glucose-responsive GAPDH gene were dramatically induced in a variety of human cells. There is a homologous palindromatic sequence GCCGTTAACGGC in the active promoter region of the two genes that is known to be required for the induction of mammalian BiP/GRP78 by A23187. The evidence confirms in general the function of this element in the regulation of calcium-associated gene activity.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Base Sequence
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Blotting, Northern
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Calcimycin / pharmacology*
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Carrier Proteins / genetics*
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Cell Line
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Endoplasmic Reticulum Chaperone BiP
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Gene Expression Regulation / drug effects*
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Glyceraldehyde-3-Phosphate Dehydrogenases / genetics*
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Heat-Shock Proteins*
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Humans
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In Vitro Techniques
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Molecular Chaperones*
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Molecular Sequence Data
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Promoter Regions, Genetic
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RNA, Messenger / genetics
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Regulatory Sequences, Nucleic Acid
Substances
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Carrier Proteins
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Endoplasmic Reticulum Chaperone BiP
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HSPA5 protein, human
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Heat-Shock Proteins
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Molecular Chaperones
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RNA, Messenger
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Calcimycin
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Glyceraldehyde-3-Phosphate Dehydrogenases