Genetic screens using the piggyBac transposon

Methods. 2011 Apr;53(4):366-71. doi: 10.1016/j.ymeth.2010.12.022. Epub 2010 Dec 23.


Transposons are an attractive system to use in genetic screens as they are molecularly tractable and the disrupted loci that give rise to the desired phenotype are easily mapped. We consider herein the characteristics of the piggyBac transposon system in complementing existing mammalian screen strategies, including the Sleeping Beauty transposon system. We also describe the design of the piggyBac resources that we have developed for both forward and reverse genetic screens, and the protocols we use in these experiments.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Coculture Techniques
  • DNA Mutational Analysis / methods*
  • DNA Transposable Elements*
  • Embryonic Stem Cells
  • Gene Components
  • Genes, Neoplasm
  • Genetic Vectors
  • Humans
  • Hybridization, Genetic
  • Kaplan-Meier Estimate
  • Mice
  • Polymerase Chain Reaction / methods
  • Sequence Analysis, DNA / methods
  • Transfection / methods
  • Transposases / genetics


  • DNA Transposable Elements
  • Transposases
  • sleeping beauty transposase, human