Whole gene amplification and protein separation from a few cells

Anal Biochem. 2011 Apr 1;411(1):64-70. doi: 10.1016/j.ab.2010.12.028. Epub 2010 Dec 24.

Abstract

Despite the growing interest to explore untapped microbial gene and protein diversity, no single platform has been able to acquire both gene and protein information from just a few cells. We present a microfluidic system that simultaneously performs on-chip capillary electrophoresis for protein analysis and whole genome amplification (WGA), and we demonstrate this by doing both for the same cohort of cyanobacterial cells. This technology opens avenues for studying protein profiles of precious environmental microbial samples and simultaneously accessing genomic information based on WGA.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / isolation & purification*
  • Genes, Bacterial / genetics*
  • Microfluidics
  • Nucleic Acid Amplification Techniques / methods*
  • Synechococcus / cytology*
  • Synechococcus / genetics*

Substances

  • Bacterial Proteins