Coomassie staining as loading control in Western blot analysis

J Proteome Res. 2011 Mar 4;10(3):1416-9. doi: 10.1021/pr1011476. Epub 2011 Feb 3.


In Western blotting, immunodetection of housekeeping proteins is routinely performed to detect differences in electrophoresis loading. The present work describes a much faster and simpler protein staining method, which is compatible with ordinary blocking conditions. In addition, the method can be used after immunodetection with superior linearity compared to ordinary staining methods. After immunoblotting and staining, protein bands can be further identified using peptide mass fingerprinting.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blotting, Western / methods*
  • Peptide Mapping / methods
  • Proteins / analysis*
  • Rosaniline Dyes / chemistry*
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods
  • Staining and Labeling / methods*


  • Proteins
  • Rosaniline Dyes
  • coomassie Brilliant Blue