A critical review of permeabilized cell systems for studying mammalian DNA repair

Mutat Res. 1990 Sep-Nov;236(2-3):239-52. doi: 10.1016/0921-8777(90)90008-s.

Abstract

Permeabilized cell systems have proven valuable for studies of the enzymology of mammalian DNA repair and this review will summarize and contrast the different systems used to this end. Results from permeable cell studies will be reviewed which pertain to 3 questions of DNA repair: the role(s) of ATP, DNA polymerase enzymology, and the isolation of repair factors by in vitro correction of repair-defective cells.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.
  • Review

MeSH terms

  • Adenosine Triphosphate / physiology
  • Animals
  • Aphidicolin
  • Cell Membrane Permeability
  • DNA Polymerase II / antagonists & inhibitors
  • DNA Polymerase III
  • DNA Repair*
  • DNA-Directed DNA Polymerase / pharmacology
  • DNA-Directed DNA Polymerase / physiology
  • Dideoxynucleotides
  • Diterpenes / pharmacology
  • Humans
  • Kinetics
  • Mammals
  • Thymine Nucleotides / pharmacology

Substances

  • Dideoxynucleotides
  • Diterpenes
  • Thymine Nucleotides
  • Aphidicolin
  • Adenosine Triphosphate
  • DNA Polymerase II
  • DNA Polymerase III
  • DNA-Directed DNA Polymerase
  • 2',3'-dideoxythymidine triphosphate