The role of mannose-binding lectin gene polymorphisms in susceptibility to HIV-1 infection in Southern Brazilian patients

AIDS. 2011 Feb 20;25(4):411-8. doi: 10.1097/QAD.0b013e328342fef1.

Abstract

Objective: This study investigates the role of mannose-binding lectin (MBL) in the susceptibility to HIV-1 infection analyzing polymorphisms located at the MBL2 promoter and exon 1 regions.

Materials and methods: The prevalence of MBL2 variant alleles was investigated in 410 HIV-1-infected patients from the South Brazilian HIV cohort and in 345 unexposed uninfected healthy individuals. The promoter variants were genotyped using polymerase chain reaction with sequence-specific primers (PCR-SSP) and exon 1 variants were analyzed by real-time PCR using a melting temperature assay and were confirmed by PCR-restriction fragment length polymorphism (RFLP). MBL2 genotypic and allelic frequencies were compared between HIV-1-infected patients and controls using the chi-squared tests.

Results: The analyses were performed subdividing the individuals according to their ethnic origin. Among Euro-derived individuals a higher frequency of the LX/LX genotype was observed in patients when compared to controls (P < 0.001). The haplotypic analysis also showed a higher frequency of the haplotypes associated with lower MBL levels among HIV-1-infected patients (P = 0.0001). Among Afro-derived individuals the frequencies of LY/LY and HY/HY genotypes were higher in patients when compared to controls (P = 0.009 and P = 0.02).

Conclusions: An increased frequency of MBL2 genotypes associated with low MBL levels was observed in Euro-derived patients, suggesting a potential role for MBL in the susceptibility to HIV-1 infection in Euro-derived individuals.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Brazil / epidemiology
  • Female
  • Genetic Predisposition to Disease / genetics*
  • HIV Infections / epidemiology
  • HIV Infections / genetics*
  • HIV Infections / immunology
  • HIV-1*
  • Humans
  • Lectins, C-Type / genetics
  • Lectins, C-Type / immunology
  • Male
  • Mannose-Binding Lectin / genetics*
  • Mannose-Binding Lectin / immunology
  • Mannose-Binding Lectins / genetics
  • Mannose-Binding Lectins / immunology
  • Middle Aged
  • Polymorphism, Genetic
  • Promoter Regions, Genetic / genetics*
  • Receptors, Cell Surface / genetics
  • Receptors, Cell Surface / immunology
  • Young Adult

Substances

  • Lectins, C-Type
  • MBL2 protein, human
  • Mannose-Binding Lectin
  • Mannose-Binding Lectins
  • Receptors, Cell Surface
  • mannose receptor