An ELISA for the serodiagnosis of tuberculosis using a 30,000-Da native antigen of Mycobacterium tuberculosis

J Infect Dis. 1990 Oct;162(4):928-31. doi: 10.1093/infdis/162.4.928.


An ELISA was established for the measurement of IgG antibody in human serum to the 30,000-Da native antigen of Mycobacterium tuberculosis and evaluated for its utility in the diagnosis of tuberculosis at the Instituto Nacional de Enfermedades Respiratorias in Mexico City. The test had a sensitivity of 70% in patients with sputum-positive active pulmonary tuberculosis and a specificity in control subjects of 100%. The accuracy of positive prediction was 100% and of negative prediction 93% for patients with pulmonary tuberculosis at this institute. Less favorable test characteristics were obtained for patients with miliary and pleural tuberculosis, in which the test had sensitivities of 22% and 14%. These results offer the promise of an accurate serodiagnostic test for pulmonary tuberculosis using readily obtained reagents.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Antibodies, Bacterial / blood*
  • Antigens, Bacterial / immunology*
  • Enzyme-Linked Immunosorbent Assay*
  • Humans
  • Lung Diseases, Fungal / diagnosis
  • Mycobacterium tuberculosis / immunology*
  • Predictive Value of Tests
  • Tuberculosis / diagnosis*
  • Tuberculosis, Miliary / diagnosis
  • Tuberculosis, Pleural / diagnosis
  • Tuberculosis, Pulmonary / diagnosis


  • Antibodies, Bacterial
  • Antigens, Bacterial