Abstract
Influenza virosomes are virus-like particles, representing a platform for vaccine development. In this study, we examined the immunogenicity of avian influenza virosomes with or without inclusion of recombinant chicken interferon-gamma (rChIFN-γ) or CpG-ODN in chickens. Immunization with virosomes adjuvanted with CpG-ODN elicited the highest haemagglutination inhibition antibody titres, as well as IgG and IgA serum antibody responses. Moreover, Virosomes+CpG-ODN formulation induced an antigen-specific spleen cell proliferation and IFN-γ expression. In conclusion, our results demonstrated that virus-specific antibody- and cell-mediated responses may be induced in chickens immunized with virosomes and these responses can be enhanced by incorporating CpG-ODN in the virosome vaccine formulation.
Copyright © 2011 Elsevier Ltd. All rights reserved.
Publication types
-
Research Support, Non-U.S. Gov't
MeSH terms
-
Adjuvants, Immunologic / pharmacology*
-
Animals
-
Antibodies, Neutralizing / blood
-
Antibodies, Viral / blood
-
Antibody Formation
-
Chickens / immunology
-
Cross Reactions / immunology
-
Hemagglutination Inhibition Tests
-
Immunity, Cellular
-
Immunoglobulin A / blood
-
Immunoglobulin G / blood
-
Influenza Vaccines / immunology*
-
Influenza in Birds / immunology
-
Influenza in Birds / prevention & control*
-
Interferon-gamma / immunology
-
Oligodeoxyribonucleotides / pharmacology*
-
Poultry Diseases / immunology
-
Poultry Diseases / prevention & control
-
Recombinant Proteins
-
Spleen / cytology
-
Spleen / immunology
-
Vaccines, Virosome / immunology
Substances
-
Adjuvants, Immunologic
-
Antibodies, Neutralizing
-
Antibodies, Viral
-
CPG-oligonucleotide
-
Immunoglobulin A
-
Immunoglobulin G
-
Influenza Vaccines
-
Oligodeoxyribonucleotides
-
Recombinant Proteins
-
Vaccines, Virosome
-
Interferon-gamma