The role of nuclear factor-kappa-B p50 subunit in the development of endometriosis

Front Biosci (Elite Ed). 2011 Jan 1;3:591-603. doi: 10.2741/e272.

Abstract

p50 is a member of the NF-kappaB family known to be involved in endometriosis. To gain insight into the roles of p50 in the development of endometriosis, we cross-transplanted endometrial fragments from p50 knockout mice to wild-type mice and vice versa, and also autotransplanted the fragments within the knockout and wild-type mice, inducing endometriosis. We then evaluated the size of the endometrial implants, and immunoreactivity to phosphorylated p65 (p-p65), PKCepsilon and TRPV1 in ectopic and eutopic endometrium as well as in vagina. We found that p50 deletion significantly reduces the size of endometrial implants. The immunoreactivity to p-p65 and PKCepsilon, but not TRPV1, was reduced in endometrial implants in p50 knockout mice. Deletion of p50 significantly reduced p-p65 and PKCepsilon, but not TRPV1, expression in eutopic endometrium and vagina. It also disrupts NF-kappaB activation and PKCepsilon expression in eutopic and vagina, suggesting the role of NF-kappaB in regulating PKCepsilon, which plays an important role in nociception. These data show that p50 is involved in the development of endometriosis and may be a promising therapeutic target.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Endometriosis / metabolism*
  • Endometrium / metabolism
  • Endometrium / transplantation*
  • Female
  • Immunohistochemistry
  • Mice
  • Mice, Knockout
  • NF-kappa B p50 Subunit / genetics
  • NF-kappa B p50 Subunit / metabolism*
  • Protein Kinase C-epsilon / immunology
  • Protein Kinase C-epsilon / metabolism
  • Statistics, Nonparametric
  • TRPV Cation Channels / metabolism
  • Transcription Factor RelA / immunology
  • Transcription Factor RelA / metabolism

Substances

  • NF-kappa B p50 Subunit
  • Rela protein, mouse
  • TRPV Cation Channels
  • TRPV1 protein, mouse
  • Transcription Factor RelA
  • Protein Kinase C-epsilon