IgG responses to tissue-associated antigens as biomarkers of immunological treatment efficacy

J Biomed Biotechnol. 2011;2011:454861. doi: 10.1155/2011/454861. Epub 2010 Dec 19.

Abstract

We previously demonstrated that IgG responses to a panel of 126 prostate tissue-associated antigens are common in patients with prostate cancer. In the current report we questioned whether changes in IgG responses to this panel might be used as a measure of immune response, and potentially antigen spread, following prostate cancer-directed immune-active therapies. Sera were obtained from prostate cancer patients prior to and three months following treatment with androgen deprivation therapy (n = 34), a poxviral vaccine (n = 31), and a DNA vaccine (n = 21). Changes in IgG responses to individual antigens were identified by phage immunoblot. Patterns of IgG recognition following three months of treatment were evaluated using a machine-learned Bayesian Belief Network (ML-BBN). We found that different antigens were recognized following androgen deprivation compared with vaccine therapies. While the number of clinical responders was low in the vaccine-treated populations, we demonstrate that ML-BBN can be used to develop potentially predictive models.

Publication types

  • Comparative Study
  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Algorithms
  • Antigens, Neoplasm / immunology*
  • Artificial Intelligence
  • Bayes Theorem
  • Biomarkers, Tumor / blood
  • Biomarkers, Tumor / immunology*
  • Cancer Vaccines / administration & dosage
  • Cancer Vaccines / immunology
  • Computational Biology
  • Humans
  • Immunoblotting
  • Immunoglobulin G / blood
  • Immunoglobulin G / immunology*
  • Male
  • Prostatic Neoplasms / immunology*
  • Prostatic Neoplasms / metabolism
  • Prostatic Neoplasms / therapy*
  • Signal Transduction
  • Treatment Outcome
  • Vaccines, DNA / immunology

Substances

  • Antigens, Neoplasm
  • Biomarkers, Tumor
  • Cancer Vaccines
  • Immunoglobulin G
  • Vaccines, DNA