Neurofilament heavy subunit in cerebrospinal fluid: a biomarker of amyotrophic lateral sclerosis?

Amyotroph Lateral Scler. 2011 Mar;12(2):144-7. doi: 10.3109/17482968.2010.542002. Epub 2011 Jan 4.

Abstract

The objectives of this study were to investigate the presence of the three neurofilament subunits, ubiquitin, proteasome and 3-nitrotyrosine, in CSF samples of ALS patients. CSF samples were obtained by lumbar puncture from 10 ALS patients and six controls. All samples were analysed by Western blotting. Results revealed that neurofilament heavy subunit was identified in 70% of ALS cases and we conclude that this subunit may be a promising biomarker for clinical diagnosis of ALS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adult
  • Aged
  • Amyotrophic Lateral Sclerosis / cerebrospinal fluid*
  • Amyotrophic Lateral Sclerosis / diagnosis*
  • Amyotrophic Lateral Sclerosis / pathology
  • Biomarkers / cerebrospinal fluid*
  • Female
  • Humans
  • Male
  • Middle Aged
  • Neurofilament Proteins / cerebrospinal fluid*
  • Neurons / cytology
  • Neurons / metabolism
  • Protein Subunits / cerebrospinal fluid*
  • Spinal Cord / cytology
  • Spinal Cord / metabolism
  • Spinal Cord / pathology
  • Tyrosine / analogs & derivatives
  • Tyrosine / metabolism

Substances

  • Biomarkers
  • Neurofilament Proteins
  • Protein Subunits
  • 3-nitrotyrosine
  • Tyrosine