Comparison of the QIAsymphony automated nucleic acid extraction and PCR setup platforms with NucliSens easyMAG and Corbett CAS-1200 liquid handling station for the detection of enteric pathogens in fecal samples

J Microbiol Methods. 2011 Feb;84(2):335-40. doi: 10.1016/j.mimet.2010.12.024. Epub 2010 Dec 31.

Abstract

In this study we compared the QIAsymphony Sample Preparation and Assay Setup modules (Qiagen), which provide automated nucleic acid extraction and PCR setup, to the NucliSens easyMAG (bioMérieux) and CAS-1200 liquid handling station (Corbett) for a molecular screening approach of enteric pathogens in fecal samples using multiplex real-time PCR. The relative DNA recovery of both platforms, within- and between-run reproducibility and a prospective study, including 510 clinical fecal samples, were performed. The results demonstrated that the QIAsymphony Sample Preparation and Assay Setup modules were highly reproducible and achieve equal performance, quantitative and qualitative, when compared with the NucliSens easyMAG and CAS-1200 systems for the molecular screening analysis of enteric pathogens in fecal samples.

Publication types

  • Comparative Study
  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Automation / methods
  • Bacteriological Techniques / methods*
  • DNA, Bacterial / genetics*
  • DNA, Bacterial / isolation & purification*
  • Enterobacteriaceae / classification*
  • Enterobacteriaceae / genetics
  • Enterobacteriaceae / isolation & purification*
  • Feces / microbiology*
  • Humans
  • Mass Screening / methods
  • Polymerase Chain Reaction / methods*
  • Reagent Kits, Diagnostic
  • Reproducibility of Results

Substances

  • DNA, Bacterial
  • Reagent Kits, Diagnostic