Characterization and gene cloning of neurotactin, a Drosophila transmembrane protein related to cholinesterases

EMBO J. 1990 Nov;9(11):3593-601.

Abstract

Monoclonal antibodies have served to characterize neurotactin, a novel Drosophila protein for which a role in cell adhesion is postulated. Neurotactin is a transmembrane protein, as indicated by epitope mapping and amino acid sequence. Similarly to other cell adhesion molecules, neurotactin accumulates in parts of the membrane where neurotactin-expressing cells contact each other. The protein is only detected during cell proliferation and differentiation, and it is found mainly in neural tissue and also in mesoderm and imaginal discs. Neurotactin has a large cytoplasmic domain rich in charged residues and an extracellular domain similar to cholinesterase that lacks the active site serine required for esterase activity. The extracellular domain also contains three copies of the tripeptide leucine-arginine-glutamate, a motif that forms the primary sequence of the adhesive site of vertebrate s-laminin.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Cell Adhesion Molecules / genetics*
  • Cell Adhesion Molecules / immunology
  • Cell Adhesion Molecules / metabolism
  • Cholinesterases / genetics
  • Cholinesterases / immunology
  • Cholinesterases / metabolism
  • Chromosome Mapping
  • Cloning, Molecular
  • DNA / genetics
  • Drosophila Proteins*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Gene Expression
  • Immunoenzyme Techniques
  • Membrane Glycoproteins / genetics*
  • Membrane Glycoproteins / immunology
  • Membrane Glycoproteins / metabolism
  • Molecular Sequence Data
  • Transcription, Genetic

Substances

  • Cell Adhesion Molecules
  • Drosophila Proteins
  • Membrane Glycoproteins
  • Nrt protein, Drosophila
  • DNA
  • Cholinesterases

Associated data

  • GENBANK/X53837