Novel proteins mediate an interaction between clathrin-coated vesicles and polymerizing actin filaments

Eur J Biochem. 1990 Sep 11;192(2):291-8. doi: 10.1111/j.1432-1033.1990.tb19226.x.

Abstract

A monoclonal antibody, A-7C11, was generated which reacts with two polypeptides of 40 kDa and 80 kDa associated with the coat proteins of purified brain clathirn-coated vesicles. The 40-kDa antigen was purified and found to display actin-binding properties. Negative-staining electron microscopy showed that one of the antigens reactive with A-7C11 appears to mediate the association of isolated clathrin-coated vesicles with assembling actin filaments in vitro. Immunofluorescence microscopy of cultured fibroblasts with A-7C11 revealed the antigens aligned with both actin filaments and as punctate structures near the plasma membrane. The data suggest that the interaction between clathrin-coated vesicles and the actin cytoskeleton is mediated by antigens identified by monoclonal antibody A-7C11.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Actins / metabolism*
  • Actins / ultrastructure
  • Animals
  • Antibodies, Monoclonal
  • Brain / metabolism*
  • Cattle
  • Cell Line
  • Clathrin / metabolism*
  • Coated Pits, Cell-Membrane / metabolism*
  • Coated Pits, Cell-Membrane / ultrastructure
  • Electrophoresis, Polyacrylamide Gel
  • Macromolecular Substances
  • Membrane Proteins / isolation & purification
  • Membrane Proteins / metabolism*
  • Molecular Weight
  • Muscles / metabolism*

Substances

  • Actins
  • Antibodies, Monoclonal
  • Clathrin
  • Macromolecular Substances
  • Membrane Proteins