Genomic analysis by oligonucleotide array Comparative Genomic Hybridization utilizing formalin-fixed, paraffin-embedded tissues

Methods Mol Biol. 2011:700:185-98. doi: 10.1007/978-1-61737-954-3_13.

Abstract

Formalin fixation has been used to preserve tissues for more than a hundred years, and there are currently more than 300 million archival samples in the United States alone. The application of genomic protocols such as high-density oligonucleotide array Comparative Genomic Hybridization (aCGH) to formalin-fixed, paraffin-embedded (FFPE) tissues, therefore, opens an untapped resource of available tissues for research and facilitates utilization of existing clinical data in a research sample set. However, formalin fixation results in cross-linking of proteins and DNA, typically leading to such a significant degradation of DNA template that little is available for use in molecular applications. Here, we describe a protocol to circumvent formalin fixation artifact by utilizing enzymatic reactions to obtain quality DNA from a wide range of FFPE tissues for successful genome-wide discovery of gene dosage alterations in archival clinical samples.

MeSH terms

  • Comparative Genomic Hybridization*
  • Formaldehyde / chemistry*
  • Genome-Wide Association Study / methods*
  • Humans
  • Paraffin Embedding*
  • Tissue Fixation*

Substances

  • Formaldehyde