Protection of cellular DNA and membrane from γ-radiation-induced damages and enhancement in DNA repair by sesamol

Cancer Biother Radiopharm. 2010 Dec;25(6):629-35. doi: 10.1089/cbr.2010.0803.

Abstract

Sesamol (SM), a nutritional phenolic antioxidant compound present in sesame seeds, protected pBR 322 DNA from gamma radiation-induced damages. SM prevented gamma radiation-induced degradation of covalently closed circular form of plasmid DNA in a concentration-dependent manner. Also SM protected cellular DNA of mouse blood leukocytes exposed to 4 Gy gamma radiation, ex vivo, as revealed by the data from alkaline comet assay studies. SM (5 mM) showed a faster time-dependant decrease of the radiation-induced DNA damage in mouse blood leukocytes following postirradiation incubation ex vivo, which could be attributed to enhanced DNA repair. SM protected the biomembranes from radiation-induced lipid peroxidation. Thus, SM could act as a radioprotector for the biomembranes and cellular DNA against the deleterious effects of ionizing radiation.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Benzodioxoles / pharmacology*
  • Cell Membrane / drug effects*
  • Cell Membrane / radiation effects*
  • DNA / drug effects
  • DNA / radiation effects
  • DNA Breaks, Double-Stranded / drug effects
  • DNA Damage / drug effects*
  • DNA Damage / radiation effects
  • DNA Repair / drug effects*
  • DNA Repair / radiation effects
  • Dose-Response Relationship, Drug
  • Gamma Rays*
  • Leukocytes, Mononuclear / drug effects
  • Leukocytes, Mononuclear / radiation effects
  • Lipid Peroxidation / drug effects
  • Lipid Peroxidation / radiation effects
  • Liver / drug effects
  • Liver / metabolism
  • Liver / radiation effects
  • Malondialdehyde / metabolism
  • Membrane Lipids / radiation effects
  • Mice
  • Mice, Inbred Strains
  • Nucleic Acid Conformation / radiation effects
  • Phenols / pharmacology*
  • Plasmids / drug effects
  • Plasmids / radiation effects
  • Radiation-Protective Agents / pharmacology*

Substances

  • Benzodioxoles
  • Membrane Lipids
  • Phenols
  • Radiation-Protective Agents
  • Malondialdehyde
  • DNA
  • sesamol