A Scalable, Fully Automated Process for Construction of Sequence-Ready Human Exome Targeted Capture Libraries

Genome Biol. 2011;12(1):R1. doi: 10.1186/gb-2011-12-1-r1. Epub 2011 Jan 4.

Abstract

Genome targeting methods enable cost-effective capture of specific subsets of the genome for sequencing. We present here an automated, highly scalable method for carrying out the Solution Hybrid Selection capture approach that provides a dramatic increase in scale and throughput of sequence-ready libraries produced. Significant process improvements and a series of in-process quality control checkpoints are also added. These process improvements can also be used in a manual version of the protocol.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Automation, Laboratory*
  • Exome*
  • Gene Library*
  • Genome, Human
  • High-Throughput Nucleotide Sequencing
  • Humans
  • Nucleic Acid Hybridization / methods*
  • Oligonucleotide Array Sequence Analysis / methods*
  • Quality Control