Sequence analysis of Enterococcus faecalis aggregation substance encoded by the sex pheromone plasmid pAD1

Mol Microbiol. 1990 Jun;4(6):895-904. doi: 10.1111/j.1365-2958.1990.tb00662.x.


The location of the structural gene for aggregation substance on the sex pheromone plasmid pAD1 of Enterococcus faecalis was determined using an oligonucleotide deduced from the N-terminal amino acid sequence of the purified protein. The nucleotide sequence was determined for the corresponding region and two open reading frames (ORFs) could be identified. ORF1 codes for a small (Mr 13,160) acidic protein of unknown function. The gene for aggregation substance (named asa1) was found to code for a protein of 1296 amino acids (Mr 142,248). The protein has a signal peptide of 43 amino acids (the resulting Mr for mature aggregation substance is 137,429) and contains in its C-terminal region a proline-rich sequence, previously characterized as being involved in cell wall association, which is followed by a membrane anchor. The membrane anchor showed significant similarity to that of other Gram-positive organisms, but no other similarities to surface proteins from Gram-positive bacteria were found. In particular, no repeats on the DNA or protein level could be detected for pAD1-specific aggregation substance. The protein contains the amino acid motifs Arg-Gly-Asp-Ser and Arg-Gly-Asp-Val (once each), which, it is proposed, play a crucial role in adherence to eukaryotic cells.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Bacterial Adhesion
  • Bacterial Proteins / biosynthesis
  • Bacterial Proteins / genetics*
  • Base Sequence
  • DNA, Bacterial / analysis
  • Enterococcus faecalis / genetics*
  • Molecular Sequence Data
  • Plasmids / genetics*
  • Sequence Homology, Nucleic Acid
  • Sex Attractants / biosynthesis
  • Sex Attractants / genetics*


  • Bacterial Proteins
  • DNA, Bacterial
  • Sex Attractants
  • aggregation substance, Enterococcus faecalis

Associated data

  • GENBANK/X17214