Liquid Chromatography-Mass Spectrometry (LC-MS)-based Lipidomics for Studies of Body Fluids and Tissues

Methods Mol Biol. 2011;708:247-57. doi: 10.1007/978-1-61737-985-7_15.

Abstract

In this paper, analytical methodologies for the global profiling of lipids in serum and tissue samples are reported. The sample preparation is based on a modified Folch extraction, and the analysis is carried out with ultrahigh-performance liquid chromatography combined with mass spectrometry (UPLC-MS). For further identification, MS(n) mass spectrometry is carried out utilizing an LTQ-Orbitrap mass spectrometry as the detector. Such a system affords determination of accurate masses and is thus a highly useful tool for lipid identification. The repeatability of the analysis proved to be good, with relative standard errors for spiked samples being between 4.51 and 10.44%. The throughput of the methodology described here is over 100 samples a day.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Analytic Sample Preparation Methods
  • Body Fluids / chemistry*
  • Chromatography, High Pressure Liquid / methods*
  • Clinical Chemistry Tests / methods*
  • Humans
  • Lipids / analysis*
  • Lipids / blood
  • Lipids / isolation & purification
  • Mass Spectrometry / methods*

Substances

  • Lipids