Electrospray Ionization Tandem Mass Spectrometry (ESI-MS/MS)-based Shotgun Lipidomics

Methods Mol Biol. 2011;708:259-75. doi: 10.1007/978-1-61737-985-7_16.

Abstract

In the past decade, many new strategies for mass spectrometry (MS)-based analyses of lipids have been developed. Lipidomics is one of the most promising research fields to emerge as a result of these advances in MS. Currently, mass spectrometric analysis of lipids involves two complementary approaches: direct infusion (shotgun lipidomics) and liquid chromatography coupled to MS. In this chapter, I will demonstrate the approach of shotgun lipidomics using electrospray ionization tandem MS for the analysis of lipid molecular species directly from crude biological extracts of tissue or fluids.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Animals
  • Cattle
  • Chemical Fractionation
  • Lipids / analysis*
  • Lipids / blood
  • Lipids / chemistry
  • Lipids / isolation & purification
  • Liver / chemistry
  • Reference Standards
  • Solvents / chemistry
  • Spectrometry, Mass, Electrospray Ionization / methods*
  • Spectrometry, Mass, Electrospray Ionization / standards
  • Tandem Mass Spectrometry / methods*
  • Tandem Mass Spectrometry / standards

Substances

  • Lipids
  • Solvents