Direct interactions between talin and actin

Biochem Biophys Res Commun. 1990 Sep 28;171(3):1217-23. doi: 10.1016/0006-291x(90)90815-5.


Talin was purified from chicken gizzard by a modification of the method of L. Molony et al. [J. Biol. Chem.(1987) 262, 7790-7795]. Unlike the talin purified by the previous method, the talin purified by the new method was found to bind to both F- and G-actin: Talin cosedimented with F-actin. On gel filtration of a mixture of talin and G-actin, a complex of talin and action was obtained. Talin stimulated the polymerization rate of G-actin. A major proteolytic fragment of talin that retained the binding ability to F-actin was also identified. These results indicate that talin can bind directly to actin and suggest that talin plays a key role in the organization of actin filaments at the actin-membrane attachment sites in vivo also.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Actins / metabolism*
  • Animals
  • Chickens
  • Cytoskeletal Proteins / isolation & purification
  • Cytoskeletal Proteins / metabolism*
  • Gizzard, Avian / metabolism
  • Kinetics
  • Macromolecular Substances
  • Membrane Proteins / metabolism
  • Molecular Weight
  • Muscle, Smooth / metabolism
  • Protein Binding
  • Talin


  • Actins
  • Cytoskeletal Proteins
  • Macromolecular Substances
  • Membrane Proteins
  • Talin