Antimicrobial activity of Citrox bioflavonoid preparations against oral microorganisms

Br Dent J. 2011 Jan 8;210(1):E22. doi: 10.1038/sj.bdj.2010.1224.

Abstract

Background: Citrox is a formulation of soluble bioflavonoids obtained from citrus fruits. The non-toxic and antimicrobial properties of natural bioflavonoids are well documented, and consequently there has been interest in the therapeutic application of these substances.

Objective: To determine the antimicrobial activity of two Citrox formulations (BC30 and MDC30) with different bioflavonoid combinations against a range of oral microorganisms.

Methods: The antimicrobial activity of both formulations was tested against 14 bacterial species and six Candida species. The two Citrox formulations (dilution range 0.007-8% v/v) were firstly evaluated by determining the in vitro Minimal Inhibitory Concentration (MIC) against planktonic microorganisms in a broth microdilution assay. Secondly, the ability of the same serial dilutions to inhibit microbial growth was assessed in a modified microtitre biofilm assay.

Results: Both Citrox formulations exhibited antimicrobial activity. The BC30 formulation demonstrated greater activity than MDC30 and significantly inhibited growth of all bacterial species and most candidal species tested at a concentration of 1% (v/v) in both the broth and the biofilm assay.

Conclusion: Bioflavonoid preparations of Citrox have a broad-spectrum of antimicrobial activity against oral microorganisms, and as such have the potential to be used within therapeutic preparations for the control of the oral microflora.

Publication types

  • Comparative Study

MeSH terms

  • Anti-Infective Agents, Local / chemistry
  • Anti-Infective Agents, Local / therapeutic use
  • Bacteria / drug effects
  • Biofilms / drug effects*
  • Candida / drug effects
  • Citrus
  • Colony Count, Microbial
  • Dose-Response Relationship, Drug
  • Drug Combinations
  • Flavonoids / chemistry
  • Flavonoids / therapeutic use*
  • Microbial Sensitivity Tests
  • Mouth / microbiology*
  • Mouth Diseases / microbiology*
  • Mouth Diseases / prevention & control
  • Mouthwashes / chemistry
  • Mouthwashes / therapeutic use*
  • Phytotherapy
  • Plant Extracts / chemistry
  • Plant Extracts / therapeutic use

Substances

  • Anti-Infective Agents, Local
  • Drug Combinations
  • Flavonoids
  • Mouthwashes
  • Plant Extracts