Ca²+ buffering at a drosophila larval synaptic terminal

Synapse. 2011 Jul;65(7):687-93. doi: 10.1002/syn.20909. Epub 2011 Mar 10.


A quantitative analysis of Ca²+ dynamics requires knowledge of the Ca²+-binding ratio (κ(S) ); this has not been measured at Drosophila synaptic terminals or any invertebrate synaptic terminal. We measured κ(S) at a Ib motor terminal in Drosophila larvae comparing single-AP Ca²+ transients in synaptic terminals that contained varying concentrations of the Ca²+ indicator, Oregon Green 488 BAPTA-1 (OGB-1). Using a linear single-compartment model, κ(S) was calculated based upon the effect of [OGB-1] on the time constant (τ(decay) ) for the decay of intracellular free Ca²+ concentration ([Ca²+](i)). This gave a κ(S) of 77 indicating that nearly 99% of entering Ca²+ is immediately bound by endogenous fast Ca²+ buffers. Extrapolation to zero [OGB-1] gave a τ(decay) of 46 ms and a Ca²+-removal rate constant of 1641 s⁻¹ for single APs. We calculated that a single AP produced an increase in [Ca²+](i) of 196 nM and an increase in the total intracellular [Ca²+](free + bound) of 15.3 μM for measurements made in 1.0 mM external Ca²+. The increase in [Ca²+](i) for AP trains was 185 nM/ 10 Hz; this gave a Ca²+ extrusion rate constant of 827 s⁻¹, which likely reflects the activity of the plasma membrane Ca²+ ATPase. Experiments were performed to examine the effect of altering external Ca²+ or Mg²+ on Ca²+ influx at these terminals.

Publication types

  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Action Potentials / physiology
  • Animals
  • Buffers
  • Calcium / metabolism*
  • Calcium Signaling / physiology*
  • Drosophila melanogaster / physiology*
  • Larva
  • Presynaptic Terminals / physiology*


  • Buffers
  • Calcium