Dexamethasone-mediated Induction of Mouse Mammary Tumor Virus RNA: A System for Studying Glucocorticoid Action

Cell. 1975 Nov;6(3):299-305. doi: 10.1016/0092-8674(75)90181-6.

Abstract

We have investigated the mechanisms by which dexamethasone (a synthetic glucocorticoid) stimulates the production of mouse mammary tumor virus (MMTV) by cell cultures derived from mammary carcinomas of GR mice. Treatment of these cells with dexamethasone stimulates a rapid accumulation of intracellular virus-specific RNA which is dependent upon RNA synthesis but not upon DNA or protein synthesis. The effect of dexamethasone is probably mediated by a specific and saturable glucocorticoid receptor. We conclude that the accumulation of MMTV RNA is a primary response to dexamethasone and that the rate of synthesis of MMTV RNA is probably accelerated by treatment with dexamethasone.

MeSH terms

  • Cell Line
  • Cycloheximide / pharmacology
  • DNA, Viral / biosynthesis
  • Dactinomycin / pharmacology
  • Dexamethasone / antagonists & inhibitors
  • Dexamethasone / pharmacology*
  • Kinetics
  • Mammary Tumor Virus, Mouse / genetics*
  • RNA, Viral / biosynthesis*
  • Receptors, Glucocorticoid / drug effects
  • Virus Replication / drug effects

Substances

  • DNA, Viral
  • RNA, Viral
  • Receptors, Glucocorticoid
  • Dactinomycin
  • Dexamethasone
  • Cycloheximide