Proteomic detection of cancer in asbestosis patients using SELDI-TOF discovered serum protein biomarkers

Biomarkers. 2011 Mar;16(2):181-91. doi: 10.3109/1354750X.2010.543289. Epub 2011 Jan 14.

Abstract

Objectives: To identify biomarkers for cancer in asbestosis patients.

Methods: SELDI-TOF and CART were used to identify serum biomarker profiles in 35 asbestosis patients who subsequently developed cancer and 35 did not develop cancer.

Results: Three polypeptide peaks (5707.01, 6598.10, and 20,780.70 Da) could predict the development of cancer with 87% sensitivity and 70% specificity. The first two peaks were identified as KIF18A and KIF5A, respectively, and are part of the Kinesin Superfamily of proteins.

Conclusions: We identified two Kinesin proteins that can be potentially used as blood biomarkers to identify asbestosis patients at risk of developing lung cancer.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Adenocarcinoma / blood
  • Adenocarcinoma / etiology
  • Adenocarcinoma / physiopathology
  • Adenocarcinoma of Lung
  • Asbestosis / blood*
  • Asbestosis / complications
  • Asbestosis / physiopathology
  • Biomarkers, Tumor / blood*
  • Blood Proteins / analysis*
  • Case-Control Studies
  • Cohort Studies
  • Female
  • Finland
  • Humans
  • Kinesins / blood*
  • Longitudinal Studies
  • Lung Neoplasms / blood*
  • Lung Neoplasms / etiology
  • Lung Neoplasms / physiopathology
  • Male
  • Middle Aged
  • Proteomics
  • Risk Factors
  • Sensitivity and Specificity
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization

Substances

  • Biomarkers, Tumor
  • Blood Proteins
  • KIF5A protein, human
  • KIF18A protein, human
  • Kinesins