Independent encoding of grating motion across stationary feature maps in primary visual cortex visualized with voltage-sensitive dye imaging

Abstract

In early visual cortex different stimulus parameters are represented in overlaid feature maps. Such functioning was extensively explored by the use of drifting gratings characterized by orientation, spatial-temporal frequency, and direction of motion. However surprisingly, the direct cortical visuotopic drift of the gratings' stripy pattern has never been detected simultaneously to these stationary feature maps. It therefore remains to be demonstrated how physical signals of grating motion across the cortex are represented independently of other parametric maps and thus, how multi-dimensional input is processed independently to enable effective read-out further downstream. Taking advantage of the high spatial and temporal resolution of voltage-sensitive dye imaging, we here show the real-time encoding of position and orientation. By decomposing the cortical responses to drifting gratings we visualize the typical emergence of stationary orientation maps in which specific domains exhibited highest amplitudes. Simultaneously to these patchy maps, we demonstrate coherently propagating waves of activity that precisely matched the actual movement of the gratings in space and time, most dominantly for spatial frequencies lower than the preferred range. Thus, the primary visual cortex multiplexes information about retinotopic motion by additional temporal modulation of stationary orientation signals. These signals may be used to variably extract coarse-grained object motion and form information at higher visual processing stages.