Surrogate Antigens as Targets for Proteome-Wide Binder Selection

N Biotechnol. 2011 Jul;28(4):302-11. doi: 10.1016/j.nbt.2010.12.005. Epub 2011 Jan 11.

Abstract

In the past decade, many initiatives were taken for the development of antibodies for proteome-wide studies, as well as characterisation and validation of clinically relevant disease biomarkers. Phage display offers many advantages compared to antibody generation by immunisation because it is an unlimited resource of affinity reagents without batch-to-batch variation and is also amendable for high throughput in contrast to conventional hybridoma technology. One of the major bottlenecks to proteome-wide binder selection is the limited supply of suitable target antigens representative of the human proteome. Here, we provide proof of principle of using easily accessible, cancer-associated protein epitope signature tags (PrESTs), routinely generated within the Human Protein Atlas project, as surrogate antigens for full-length proteins in phage selections for the retrieval of target-specific binders. These binders were subsequently tested in western blot, immunohistochemistry and protein microarray application to demonstrate their functionality.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Antigens / immunology*
  • Binding Sites
  • Biomarkers*
  • Immunoassay / methods*
  • Protein Binding
  • Proteome / immunology*

Substances

  • Antigens
  • Biomarkers
  • Proteome