Understanding splicing regulation through RNA splicing maps

Trends Genet. 2011 Mar;27(3):89-97. doi: 10.1016/j.tig.2010.12.001. Epub 2011 Jan 12.


Alternative splicing is a highly regulated process that greatly increases the proteome diversity and plays an important role in cellular differentiation and disease. Interactions between RNA-binding proteins (RBPs) and pre-mRNA are the principle regulator of splicing decisions. Findings from recent genome-wide studies of protein-RNA interactions have been combined with assays of the global effects of RBPs on splicing to create RNA splicing maps. These maps integrate information from all pre-mRNAs regulated by single RBPs to identify the global positioning principles guiding splicing regulation. Recent studies using this approach have identified a set of positional principles that are shared between diverse RBPs. Here, we discuss how insights from RNA splicing maps of different RBPs inform the mechanistic models of splicing regulation.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Gene Expression Regulation / genetics
  • Genome-Wide Association Study
  • Humans
  • Introns / genetics
  • Models, Biological
  • RNA Splicing / genetics*
  • RNA-Binding Proteins / metabolism


  • RNA-Binding Proteins