A kaleidoscopic view of the Arabidopsis core cell cycle interactome

Trends Plant Sci. 2011 Mar;16(3):141-50. doi: 10.1016/j.tplants.2010.12.004. Epub 2011 Jan 11.

Abstract

Although protein-protein interaction (PPI) networks have been shown to offer a systems-wide view of cellular processes, only a few plant PPI maps are available. Recently, the core cell cycle of Arabidopsis thaliana has been analyzed by three independent PPI technologies, including yeast two-hybrid systems, bimolecular fluorescence complementation and tandem affinity purification. Here, we merge the three interactomes with literature-curated and computationally predicted interactions, paving the way for a comprehensive picture of the plant core cell cycle machinery. Platform-specific interactions unveil the strengths and weaknesses of each detection method and give insights into the nature of the interactions among cell cycle proteins. Moreover, comparison of the obtained data reveals that a complete interactome can only be obtained when multiple techniques are applied in parallel.

Publication types

  • Research Support, Non-U.S. Gov't
  • Review

MeSH terms

  • Arabidopsis / genetics
  • Arabidopsis / metabolism*
  • Arabidopsis Proteins / genetics
  • Arabidopsis Proteins / metabolism*
  • Cell Cycle
  • Cell Cycle Proteins / metabolism*
  • Computational Biology / methods
  • Databases, Protein
  • Microscopy, Fluorescence / methods
  • Protein Interaction Mapping / methods
  • Two-Hybrid System Techniques

Substances

  • Arabidopsis Proteins
  • Cell Cycle Proteins