Evaluation of the tissue reaction to a new bilayered collagen matrix in vivo and its translation to the clinic

Biomed Mater. 2011 Feb;6(1):015010. doi: 10.1088/1748-6041/6/1/015010. Epub 2011 Jan 17.


This study evaluates a new collagen matrix that is designed with a bilayered structure in order to promote guided tissue regeneration and integration within the host tissue. This material induced a mild tissue reaction when assessed in a murine model and was well integrated within the host tissue, persisting in the implantation bed throughout the in vivo study. A more porous layer was rapidly infiltrated by host mesenchymal cells, while a layer designed to be a barrier allowed cell attachment and host tissue integration, but at the same time remained impermeable to invading cells for the first 30 days of the study. The tissue reaction was favorable, and unlike a typical foreign body response, did not include the presence of multinucleated giant cells, lymphocytes, or granulation tissue. In the context of translation, we show preliminary results from the clinical use of this biomaterial applied to soft tissue regeneration in the treatment of gingival tissue recession and exposed roots of human teeth. Such a condition would greatly benefit from guided tissue regeneration strategies. Our findings demonstrate that this material successfully promoted the ingrowth of gingival tissue and reversed gingival tissue recession. Of particular importance is the fact that the histological evidence from these human studies corroborates our findings in the murine model, with the barrier layer preventing unspecific tissue ingrowth, as the scaffold becomes infiltrated by mesenchymal cells from adjacent tissue into the porous layer. Also in the clinical situation no multinucleated giant cells, no granulation tissue and no evidence of a marked inflammatory response were observed. In conclusion, this bilayered matrix elicits a favorable tissue reaction, demonstrates potential as a barrier for preferential tissue ingrowth, and achieves a desirable therapeutic result when applied in humans for soft tissue regeneration.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Collagen / adverse effects
  • Collagen / chemistry*
  • Female
  • Foreign-Body Reaction / pathology
  • Guided Tissue Regeneration, Periodontal / methods*
  • Humans
  • Male
  • Materials Testing
  • Mice
  • Microscopy, Electron, Scanning
  • Neovascularization, Physiologic
  • Pilot Projects
  • Porosity
  • Tissue Scaffolds / adverse effects
  • Tissue Scaffolds / chemistry*


  • Collagen