RNAi screening for fat regulatory genes with SRS microscopy

Nat Methods. 2011 Feb;8(2):135-8. doi: 10.1038/nmeth.1556. Epub 2011 Jan 16.


Identification of genes regulating fat accumulation is important for basic and medical research; genetic screening for those genes in Caenorhabditis elegans, a widely used model organism, requires in vivo quantification of lipids. We demonstrated RNA interference screening based on quantitative imaging of lipids with label-free stimulated Raman scattering (SRS) microscopy, which overcomes major limitations of coherent anti-Stokes Raman scattering microscopy. Our screening yielded eight new genetic regulators of fat storage.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Caenorhabditis elegans / chemistry
  • Caenorhabditis elegans / genetics*
  • Caenorhabditis elegans / metabolism
  • Fats / analysis*
  • Fats / metabolism
  • Gene Expression Regulation*
  • Microscopy / instrumentation
  • Microscopy / methods*
  • RNA Interference*
  • Spectrum Analysis, Raman / instrumentation
  • Spectrum Analysis, Raman / methods*


  • Fats