Nanotopographic Control of Neuronal Polarity

Nano Lett. 2011 Feb 9;11(2):505-11. doi: 10.1021/nl103349s. Epub 2011 Jan 11.

Abstract

We employ simple geometrical rules to design a set of nanotopographies able to interfere with focal adhesion establishment during neuronal differentiation. Exploiting nanoimprint lithography techniques on cyclic-olefin-copolymer films, we demonstrate that by varying a single topographical parameter the orientation and maturation of focal adhesions can be finely modulated yielding independent control over the final number and the outgrowth direction of neurites. Taken together, this report provides a novel and promising approach to the rational design of biocompatible textured substrates for tissue engineering applications.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Cell Culture Techniques / instrumentation*
  • Cell Enlargement
  • Cell Polarity
  • Crystallization / methods
  • Equipment Design
  • Equipment Failure Analysis
  • Nanostructures / chemistry*
  • Nanostructures / ultrastructure*
  • Nanotechnology / instrumentation*
  • Neurons / cytology*
  • Neurons / physiology*
  • PC12 Cells
  • Particle Size
  • Rats
  • Tissue Engineering / instrumentation*