Virological and serological surveillance for type A influenza in the black-legged kittiwake (Rissa tridactyla)

Virol J. 2011 Jan 17;8:21. doi: 10.1186/1743-422X-8-21.

Abstract

Background: The epidemiology of avian influenza viruses (AIVs) in gulls is only partially known. The role of the world's most numerous gull species, the black-legged kittiwake (Rissa tridactyla), as a potential AIV reservoir species has been unclear. The prevalence of AIV and humoral response against AIV were therefore studied in a colony of apparently healthy black-legged kittiwakes breeding in a nesting cliff in the South West Barents Region of Norway (70°22' N, 31°10' E), in 2008 and 2009.

Results: AIVs were detected from the oropharynx and cloaca in low amounts, with prevalences of 15% and 5%, in 2008 and 2009, respectively. Direct, partial sequencing of the hemagglutinin (HA) gene revealed that the H4 subtype was present. In 2009, antibodies to influenza A virus were detected in sera from 57 of 80 adult birds. In contrast, none of the three-week-old chicks (n = 18) tested seropositive. Hemagglutination inhibition (HI) assays demonstrated that the adult kittiwakes primarily had antibodies specific to the gull-associated H13 and H16 subtypes, with antibodies to H16 being most common.

Conclusions: These results support that the highly pelagic black-legged kittiwake is a reservoir of AIV. The serological findings suggest that H16 might be the main AIV subtype in the black-legged kittiwake. Further studies are needed to understand the ecology of AIV in the black-legged kittiwake and in gulls in general.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Antibodies, Viral / blood*
  • Charadriiformes / virology*
  • Cloaca / virology
  • Hemagglutination Inhibition Tests
  • Hemagglutinin Glycoproteins, Influenza Virus / genetics
  • Influenza A virus / immunology*
  • Influenza A virus / isolation & purification*
  • Influenza in Birds / epidemiology*
  • Influenza in Birds / immunology
  • Influenza in Birds / virology
  • Norway / epidemiology
  • Oropharynx / virology
  • Prevalence
  • Sequence Analysis, DNA

Substances

  • Antibodies, Viral
  • Hemagglutinin Glycoproteins, Influenza Virus