X:A ratio, the primary sex-determining signal in Drosophila, is transduced by helix-loop-helix proteins

Cell. 1990 Dec 21;63(6):1179-91. doi: 10.1016/0092-8674(90)90414-a.

Abstract

Drosophila determines its sex by "counting" X chromosomes. We show that premature expression of the pair-rule segmentation gene hairy interferes with this process, resulting in female-specific lethality by inhibiting initiation of the master control gene Sex-lethal (Sxl). The female-specific lethality can be suppressed by a constitutive Sxl allele or by extra copies of X-linked "counting elements." These results are best explained by competition between hairy and other helix-loop-helix transcription factors that act in chromosome counting. We have confirmed this model by showing that misexpression of the achaete-scute T4 gene induces ectopic Sxl expression and male-specific lethality, confirming that achaete-scute T4 is the sisterless-b counting element. We propose that X chromosomes are counted through heterodimers of helix-loop-helix transcription factors that act synergistically to initiate Sxl expression.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Blastoderm / physiology
  • Crosses, Genetic
  • DNA-Binding Proteins / metabolism*
  • Drosophila melanogaster / embryology
  • Drosophila melanogaster / genetics*
  • Embryo, Nonmammalian / physiology
  • Female
  • Genes, Lethal
  • Male
  • Restriction Mapping
  • Sex Determination Analysis*
  • Transduction, Genetic
  • Transformation, Genetic
  • X Chromosome*

Substances

  • DNA-Binding Proteins