Evaluation of the osteogenic effect of low-level laser therapy (808 nm and 660 nm) on bone defects induced in the femurs of female rats submitted to ovariectomy

Lasers Med Sci. 2011 Jul;26(4):515-22. doi: 10.1007/s10103-010-0867-9. Epub 2011 Jan 19.


The present study aimed to evaluate the effects of LLLT (660- and 808-nm wavelengths) on the process of repairing bone defects induced in the femurs of female rats submitted to ovariectomy. Bilateral ovariectomies were performed on 18 female Wistar rats, which were divided into control and irradiated groups after the digital analysis of bone density showed decreased bone mass and after standardized drilling of the femurs. The irradiated groups received 133 J/cm(2) of AsGaAl (660-nm) and InGaAlP (880-nm) laser radiation. The animals were euthanized on days 14 and 21 after the bone defects were established. Detailed descriptive histological evaluations were performed, followed by semi-quantitative histomorphometry. The results from days 14 and 21 showed that the irradiated groups presented increased density of osteoblasts, fibroblasts, and immature osteocytes on the tissue surface compared with the control (non-irradiated) groups (p < 0.05). Additionally, inflammatory infiltrate evaluations showed that LLLT decreased the accumulation of leukocytes when compared to the control treatment (p < 0.05). We concluded that, in our experimental model, both wavelengths (660-nm and 880-nm) inhibited the inflammatory process and induced the proliferation of cells responsible for bone remodeling and repair.

Publication types

  • Evaluation Study

MeSH terms

  • Animals
  • Bone Density / radiation effects
  • Disease Models, Animal
  • Female
  • Femur / radiation effects*
  • Fibroblasts / radiation effects*
  • Low-Level Light Therapy / instrumentation*
  • Low-Level Light Therapy / methods
  • Osteogenesis*
  • Osteoporosis / prevention & control
  • Osteoporosis / radiotherapy*
  • Rats
  • Statistics, Nonparametric