Effects of nanofiber/stem cell composite on wound healing in acute full-thickness skin wounds

Tissue Eng Part A. 2011 May;17(9-10):1413-24. doi: 10.1089/ten.TEA.2010.0373. Epub 2011 Mar 4.


Acute full-thickness skin wounds (FTSW) caused by extensive burns or high-energy trauma are not adequately addressed by current clinical treatments. This study hypothesized that biomimetic nanofiber scaffolds (NFSs) functionalized with rich attachment of bone-marrow-derived mesenchymal stem cells (BM-MSCs) can promote wound healing in acute FTSW. Results in a rat model showed that both NFS and BM-MSCs contributed to the wound healing. Wounds in NFS group with a higher density of BM-MSCs achieved complete closure 8 days earlier than the control group. Implanted BM-MSCs were found to promote epithelial edge ingrowth and collagen synthesis. The colocation of BM-MSCs (tagged with quantum-dots) with the expression of keratin 10 and filaggrin indicated the participation of BM-MSCs in epidermal differentiation at early and intermediate stages under the local wounding environment. Overall, this study suggests a great potential of using NFS/BM-MSC composites for the treatment of acute FTSW.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Animals
  • Biomimetic Materials
  • Bone Marrow Cells / metabolism*
  • Burns / metabolism
  • Burns / therapy*
  • Cell Differentiation
  • Humans
  • Intermediate Filament Proteins
  • Keratin-10
  • Male
  • Mesenchymal Stem Cell Transplantation*
  • Mesenchymal Stem Cells / metabolism*
  • Nanofibers*
  • Quantum Dots
  • Rats
  • Rats, Sprague-Dawley
  • Skin / injuries*
  • Skin / metabolism
  • Transplantation, Heterologous
  • Wound Healing*


  • Intermediate Filament Proteins
  • filaggrin
  • Keratin-10