Preadipocyte factor-1 Is Associated With Metabolic Profile in Severe Obesity

J Clin Endocrinol Metab. 2011 Apr;96(4):E680-4. doi: 10.1210/jc.2010-2026. Epub 2011 Jan 20.

Abstract

Context: Dysfunctional adipose tissue has been proposed as a key pathological process linking obesity and metabolic disease. Preadipocyte factor-1 (Pref-1) has been shown to inhibit differentiation in adipocyte precursor cells and could thereby play a role in determining adipocyte size, adipose tissue functioning, and metabolic profile in obese individuals.

Objective: We hypothesized that adipose tissue from metabolically healthy obese (MHO) and matched metabolically unhealthy obese individuals would demonstrate distinct differences in relation to Pref-1 expression, adipocyte size, and inflammatory markers.

Design, setting, and patients: This was a cross-sectional study, investigating obese patients undergoing bariatric surgery at a tertiary referral centre. Patients included 12 MHO and 17 age- and body mass index-matched metabolically unhealthy obese individuals.

Main outcome measures: Pref-1, monocyte chemotactic protein-1, TNF-α, granulocyte colony-stimulating factor, IL-6, and adiponectin levels, macrophage numbers, and adipocyte size were measured in omental and subcutaneous adipose tissue.

Results: The MHO group had a lower level of Pref-1 (per 1000 adipocytes) in both subcutaneous [160 (136-177) versus 194 (153-355); P < 0.05] and omental adipose tissue [102 (32-175) versus 194 (100-350); P < 0.005]. This was associated with lower numbers of macrophages, lower levels of TNF-α, monocyte chemotactic protein-1, and granulocyte colony-stimulating factor, and higher levels of adiponectin. Omental Pref-1 showed strong correlations with adipocyte size (r = 0.67, P < 0.0005) and metabolic and adipokine parameters, including percent fatty liver (r = 0.62, P < 0.005), fasting glucose (r = 0.68, P < 0.0005), triglyceride (r = 0.60, P < 0.005), high-density lipoprotein cholesterol (r = -0.46, P < 0.05), and adiponectin (r = -0.71, P < 0.05).

Conclusion: Adipose tissue in MHO individuals had lower levels of Pref-1, a known inhibitor of preadipocyte differentiation, and a more favorable inflammatory profile. These factors may be key to protecting this subgroup of obese individuals from the adverse metabolic profile associated with excess adiposity.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adipocytes / metabolism
  • Adipocytes / pathology
  • Adult
  • Bariatric Surgery
  • Calcium-Binding Proteins
  • Case-Control Studies
  • Cell Count
  • Cell Size
  • Cross-Sectional Studies
  • Female
  • Health
  • Humans
  • Inflammation Mediators / metabolism
  • Intercellular Signaling Peptides and Proteins / analysis
  • Intercellular Signaling Peptides and Proteins / blood
  • Intercellular Signaling Peptides and Proteins / physiology*
  • Macrophages / pathology
  • Male
  • Membrane Proteins / analysis
  • Membrane Proteins / blood
  • Membrane Proteins / physiology*
  • Metabolome* / physiology
  • Middle Aged
  • Obesity, Morbid / blood
  • Obesity, Morbid / metabolism*
  • Obesity, Morbid / pathology
  • Obesity, Morbid / surgery
  • Severity of Illness Index
  • Young Adult

Substances

  • Calcium-Binding Proteins
  • DLK1 protein, human
  • Inflammation Mediators
  • Intercellular Signaling Peptides and Proteins
  • Membrane Proteins