PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss

W Zhang; Z Sun; T Sun; H Zhang

doi:10.1007/s12223-010-0097-3

PCR screening and sequence analysis of iol clusters in Lactobacillus casei strains isolated from koumiss

Folia Microbiol (Praha). 2010 Nov;55(6):603-6. doi: 10.1007/s12223-010-0097-3. Epub 2011 Jan 21.

Authors

W Zhang¹, Z Sun, T Sun, H Zhang

Affiliation

¹ Key Laboratory of Dairy Biotechnology and Engineering, Ministry of Education, School of Food Science and Engineering, Inner Mongolia Agricultural University, 010018 Huhhot, China.

PMID: 21253906
DOI: 10.1007/s12223-010-0097-3

Abstract

The iol cluster (consisting of genes involved in myo-inositol utilization) was investigated in Lactobacillus casei strains isolated from koumiss. Ten strains were tested for the presence of iol cluster by PCR screening; three strains encoded this cluster. Full-sequencing procedure was conducted; the iol cluster was identical to that of L. casei BL23 (GenBank access. no. FM177140) except for an upstream transposase. The iol cluster is not a common feature for L. casei strains isolated from koumiss.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bacteriological Techniques / methods*
Horses
Lacticaseibacillus casei / genetics*
Lacticaseibacillus casei / isolation & purification
Mass Screening / methods
Milk / microbiology*
Molecular Sequence Data
Multigene Family*
Polymerase Chain Reaction*
Sequence Analysis, DNA*

Associated data

GENBANK/FJ967835