PAS/poly-HAMP Signalling in Aer-2, a Soluble Haem-Based Sensor

Mol Microbiol. 2011 Feb;79(3):686-99. doi: 10.1111/j.1365-2958.2010.07477.x. Epub 2010 Dec 7.

Abstract

Poly-HAMP domains are widespread in bacterial chemoreceptors, but previous studies have focused on receptors with single HAMP domains. The Pseudomonas aeruginosa chemoreceptor, Aer-2, has an unusual domain architecture consisting of a PAS-sensing domain sandwiched between three N-terminal and two C-terminal HAMP domains, followed by a conserved kinase control module. The structure of the N-terminal HAMP domains was recently solved, making Aer-2 the first protein with resolved poly-HAMP structure. The role of Aer-2 in P. aeruginosa is unclear, but here we show that Aer-2 can interact with the chemotaxis system of Escherichia coli to mediate repellent responses to oxygen, carbon monoxide and nitric oxide. Using this model system to investigate signalling and poly-HAMP function, we determined that the Aer-2 PAS domain binds penta-co-ordinated b-type haem and that reversible signalling requires four of the five HAMP domains. Deleting HAMP 2 and/or 3 resulted in a kinase-off phenotype, whereas deleting HAMP 4 and/or 5 resulted in a kinase-on phenotype. Overall, these data support a model in which ligand-bound Aer-2 PAS and HAMP 2 and 3 act together to relieve inhibition of the kinase control module by HAMP 4 and 5, resulting in the kinase-on state of the Aer-2 receptor.

Publication types

  • Research Support, N.I.H., Extramural

MeSH terms

  • Adaptation, Physiological / drug effects
  • Amino Acid Sequence
  • Bacterial Proteins / chemistry*
  • Carbon Monoxide / pharmacology
  • Chemotaxis / drug effects
  • Escherichia coli / cytology
  • Escherichia coli / drug effects
  • Escherichia coli / metabolism
  • Escherichia coli Proteins / metabolism
  • Heme / metabolism*
  • Molecular Sequence Data
  • Nitric Oxide / pharmacology
  • Oxygen / pharmacology
  • Protein Binding / drug effects
  • Protein Structure, Quaternary
  • Protein Structure, Secondary
  • Protein Structure, Tertiary
  • Sequence Deletion
  • Signal Transduction* / drug effects
  • Solubility / drug effects

Substances

  • ARR-2 protein, Pseudomonas aeruginosa
  • Bacterial Proteins
  • Escherichia coli Proteins
  • Nitric Oxide
  • Heme
  • Carbon Monoxide
  • Oxygen